Portable Microelisa System for Toxicological Hair Analysis
نویسندگان
چکیده
An automated full-range quantitation method for identifying d-methamphetamine (d-MA) in human hair using a microchip-based ELISA system (microELISA) in combination with a micropulverized extraction method for sample preparation has been developed. An anti-d-MA antibody and a peroxidase-linked MA were used for the competitive ELISA assay. Method validation was carried out using doped hair samples, and segmental analyses of real-case specimens were carried out by both microELISA and liquid chromatography/tandem mass spectrometry (LC–MS/MS) to verify the reliability and applicability of this new method. Sample preparation and quantitation can be accomplished easily and quickly (less than 30 min) in smallscale contamination-free environments.
منابع مشابه
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